Isolation of high-quality RNA is one of the most crucial methods in molecular biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large amounts of polysaccharides, polyphenols and other secondary metabolites. Here we present a suitable protocol for RNA isolation from a wide range of woody plants that includes eight gymnosperms and four ...

Due to densification of wireless networks, there exist abundance of idling computation resources at (network) edge devices (e.g., access points and handheld computers). These resources can be scavenged by offloading heavy computation tasks from small IoT devices (e.g., sensors and wearable computing devices) in proximity, thereby overcoming their limitations and lengthening their battery lives....

background and objectives: infection with human immunodeficiency virus (hiv) results in dysregulation of the cytokine profile. a switch from a t helper 1 (th1) to a th2 cytokine has been proposed as an important factor in progression of hiv infection to aids. the aim of the present study was to assess the level of th1 and th2 cytokines in hiv infected individuals in order to identify the switch...

interleukin-4 (il-4) is produced by t-helper cells type 2 (th2) and induces ige synthesis. t-helper cells type 1 (th1) produce interferon-gamma (ifn-gamma) which suppresses th2 and reduces il-4 induced ige production. in this :;tudy , we demonstrated that the levels of specific ige in the serum of atopic children (n=20) were elevated while il-4 production was increased and ifn-gamma secretion w...

In this paper a novel common mode separation technique for implementing fully differential current buffers is introduced.  Using the proposed method two high CMRR (Common Mode Rejection Ratio) and high PSRR (Power Supply Rejection Ratio) fully differential current buffers in BIPOLAR and CMOS technologies are implemented.   Simulation results by HSPICE using 0.18μm TSMC process for CMOS based st...

INTRODUCTION In order to employ Nicotiana tabacum cells as a profitable natural bioreactor for production of bio-functional "Soluble human TRAIL" (ShTRAIL), endoplasmic reticulum (ER) targeted expression and innovative extraction procedures were exploited. METHODS At first, the ShTRAIL encoding gene was sub-cloned into designed H2 helper vector to equip it with potent TMV omega leader sequenc...

the cd30 antigen seems to play a costimulatory role in maintaining the physiological balance between t-helper (th)1/th2 immune responses. in this study, plasma and in vitro soluble cd30 (scd30) secretion was investigated in patients with coronary artery disease (cad) as a plausible marker of dysregulated immune response. twenty one patients with angiographically confirmed cad and 31 healthy con...